Phosphomannomutase is responsible for conversion of mannose-6-pho

Phosphomannomutase is responsible for conversion of mannose-6-phosphate to mannose-1-phosphate. Furthermore, manB is flanked by galU, a glucose pyrophosphorylase, and csrA, a putative carbon storage regulator (Table 3 and additional file 2, Figure S1). Genome annotation also identified the presence of a ~19 kb region that contains a cluster of genes predicted to encode for glycosyltransferases,

transport proteins, and other proteins involved in polysaccharide biosynthesis (Table 3 and additional click here file 2, Figure S1). The G+C content (36%) of this locus was similar to that of H. somni genomes (37%) [2, 25]. Table 3 Putative EPS genes in H.somni 2336 and 129Pt with proposed roles in polysaccharide synthesis Gene ORF (HSM-H. somni 2336 and HS- H. somni 129Pt) Protein annotation No. of amino acids, predicted mass (kDa) % Similarity to another protein galU HSM_1063 HS_1117 UTP-glucose-1-phosphate uridylyltransferase 295, 32.2 70, to glucose-1-phosphate uridylyltransferase, galU (E. coli) manB

HSM_1062 HS_1118 Phosphomannomutase 454, 50.3 81, to phosphomannomutase, cpsG (E. coli) csrA HSM_1061 HS_1119 Carbon storage regulator 60, 6.75 89, to pleiotropic regulatory protein for carbon source metabolism, csrA (E. coli) pldB HSM_1242 HS_0775 Lysophospholipase selleck kinase inhibitor 318, 37.4 49, to lysophospholipase L2, pldB (E. coli) ybhA HSM_1241 HS_0774 Haloacid dehalogenase-like hydrolase 273, 30.8 60, to phosphatase//phospho transferase, ybhA (E. coli) araD HSM_1240 HS_0773 L-ribulose-5-phosphate 4-epimerase 231, 25.8 82, to L-ribulose-5-phosphate 4-epimerase, Adenosine yiaS (E. coli) sgbU HSM_1239 HS_0772 Putative L-xylulose-5-phosphate 3-epimerase 290, 33.2 84, to Semaxanib L-xylulose 5-phosphate 3-epimerase, yiaQ (E. coli) rmpA HSM_1238 HS_0771 3-keto-L-gulonate-6-phosphate decarboxylase 215, 23.6 64, to 3-keto-L-gulonate 6-phosphate decarboxylase, yiaQ (E. coli) xylB HSM_1237 HS_0770 L-xylulose kinase 484, 53.7 75, to L-xylulose kinase, lyxK (E. coli) rbs1C HSM_1236

HS_0769 Ribose ABC transporter, permease 342, 32.9 59, to D-ribose transporter subunit, rbsc (E. coli) rbs1A HSM_1235 HS_0768 Ribose ABC transporter, ATPase component 496, 56.1 60, to D-ribose transporter subunit, ATP-binding component, rbsA (E. coli K12) rbs1B HSM_1234 HS_0767 ABC-type sugar transport system, periplasmic component 312, 31.0 56, to D-ribose transporter subunit, periplasmic component (E. coli ) glsS HSM_1233 HS_0766 Gluconolaconase 295, 32.6 46, to gluconolactonase, gnl (Zymomonas mobilis) rbs2B HSM_1232 HS_0765 ABC-type sugar-binding periplasmic protein 369, 37.2 81, to hypothetical protein (Yersinia intermedia ATCC 29909) rbs2C HSM_1231 HS_0764 Ribose ABC transporter, permease 349, 36.9 90, to inner-membrane translocator (Yersinia intermedia ATCC 29909) rbs2A HSM_1230 HS_0763 Ribose ABC transporter, ATPase component 505, 55.

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