4A). Under these experimental conditions, overexpression of VEGF for 4 weeks was associated with increased new vessel formation within the liver (Supporting Fig. 4B) and increased hepatic collagen deposition (Sirius red staining, Fig. 3A). In line with these changes, overexpression of VEGF also resulted in a time-dependent increase of hydroxyproline, a collagen-specific amino acid, and Col1a1 mRNA within the liver (Fig. 3B,C). AZD8055 purchase As depicted in Fig. 2E,F, overexpression of VEGF was also associated with altered hepatic levels of Cxc chemokines. As in CCl4-treated mice (Supporting Fig. 2), the angiogenic chemokine Cxcl1 (Supporting Fig. 4C) and the angiostatic

chemokine Cxcl9 (Fig. 3D) were highly abundant within the liver in response to VEGF overexpression. Because the in vivo results suggested a close association between VEGF pathways and the expression of chemokines, we next assessed the direct effects of the angiostatic chemokine Navitoclax nmr Cxcl9 on VEGF-mediated effects on endothelial cells and stellate cells in vitro. Both cell types are considered to be involved in neoangiogenesis within the liver 14 and express both Cxcl9 and its receptor Cxcr3 (Supporting Fig. 5A,B). As depicted in Fig. 4A,B, Cxcl9 significantly abrogated the proliferative and migratory response of VEGF on endothelial cells. We next assessed direct functional aspects of Cxcl9 on angiogenesis in a Matrigel assay. As

shown in Fig. 4C, Cxcl9 indeed strongly abrogated endothelial network formation, supporting its direct involvement in VEGF-induced vessel formation. Furthermore, Cxcl9 inhibited the scratch closure in a functional scratch assay, which is also considered as a combination of proliferation and migration of endothelial cells (Supporting Fig. 5C). Importantly, the inhibitory effects of Cxcl9 were also found in primary sinusoidal endothelial cells isolated from livers of medchemexpress CCl4 damaged animals (Fig. 5A,B), supporting the relevance of our findings for the injury model used in our study. On a molecular level, the effects of

Cxcl9 were associated with a reduced phosphorylation of VEGFR2 (KDR), its downstream mediator PLCγ, JNK, and ERK in primary endothelial cells (Fig. 5C), supporting earlier results of antiangiogenic chemokines on the VEGF signaling pathway. 17 Cxcl9 also reduced the VEGF-induced proliferation of stellate cells (Supporting Fig. 6A), which was also associated with a reduced phosphorylation of KDR, JNK, and ERK (Supporting Fig. 6B). As endothelial and stellate cells are both considered to play a pivotal role during liver neoangiogenesis, we also evaluated the direct interaction between these cell types with and without treatment of Cxcl9. Indeed, conditioned medium from VEGF stimulated endothelial cells induced the proliferation and migration of stellate cells in vitro, which was strongly reduced by concomitant treatment of endothelial cells with Cxcl9 (Supporting Fig. 6C).