03). The patients with diabetes received the same treatment for ovarian cancer as the patients without diabetes. The association of metformin use and increased progression-free survival, but not overall survival, remained significant after controlling for standard clinicopathologic
parameters.
CONCLUSION: In this ovarian cancer cohort, the patients with type II diabetes who used metformin had longer progression-free survival, despite receiving similar treatment for ovarian cancer. (Obstet Gynecol 2012;119:61-7) DOI: 10.1097/AOG.0b013e3182393ab3″
“Contents The aim of this study was to determinate the semen quality of frozenthawed samples that were chilled for up to 2 days before freezing. The MK-0518 supplier ejaculates (n = 18) from six dogs were collected, pooled and divided into six aliquots. The first aliquot (C, control) Vorinostat concentration was frozen in liquid nitrogen using a conventional protocol to reach a final concentration of 100 x 106 spermatozoa/ml, 20% egg yolk and 5% glycerol. The remaining five aliquots were diluted with
a chilled extender (Tris-glucose and 20% egg yolk) and cooled at 4 degrees C as follows: R1, the semen was cooled for 1 h; R6, the semen was cooled for 6 h; R12, the semen was cooled for 12 h; R24, the semen was cooled for 24 h and R48, the semen was cooled for 48 h. After the chilling period, a second extender was added (Tris-glucose, 20% egg yolk, 10% glycerol and Equex at 1%) to reach a final composition similar to aliquot C, and then, the semen samples (R1, R6, R12, R24 and R48) were frozen in liquid nitrogen. The post-thaw sperm quality was
assessed in 30 straws from each experimental group. After freezingthawing, the total sperm motility (approximately 60-70%) in the semen chilled for up to 48 h did not show any differences from the samples frozen by the conventional cryopreservation method (63.2%). No significant differences were detected in the percentages of abnormal sperm cells among the fresh semen, the control group and the frozen samples after AL3818 cost the different cooling times. Finally, the post-thaw percentages of damaged acrosomes showed a very uniform distribution, with mean values ranging between 7% and 10.5%. The results clearly demonstrated that cooling the semen up to 48 h before freezing did not produce a decrease in the semen quality when was compared with semen frozen by a traditional procedure.”
“Lactic cultures were screened for their ability to accumulate gamma-aminobutyric acid (GABA) in the culture medium. Lactobacillus bulgaricus CFR 2028 produced the highest yields of GABA (22.7 mM) from the substrate monosodium glutamate (MSG). Instrumental characterization by high-performance liquid chromatography and structural characterization by mass spectrometry confirmed GABA production by L. bulgaricus CFR 2028.