From our knowledge of what structural features are required to be a substrate for Pad-decarboxylation, a number of non-competitive enzyme inhibitors have been found which prevent enzyme activity and decrease mould resistance to sorbic acid. These are typically 2,4-unsaturated aldehydes that conform to the dimensions defined for substrates (Archer et al., 2008). We speculate that the chemically reactive aldehyde moiety causes enzyme damage by covalently bonding, preventing further activity.
It is interesting to note that oil of cinnamon contains not only cinnamic acid but also cinnamaldehyde (Burdock, 2002), one of the key inhibitors of Pad-decarboxylation. It appears to indicate not only synthesis of cinnamic acid by plants as an inhibitor of mould infection but also possibly the synthesis of the aldehyde to combat the mould’s mechanism LDK378 ic50 Selleckchem Galunisertib of resistance
to cinnamic acid. The following are the supplementary materials related to this article. Supplementary data, Table 1. Substrates tested for decarboxylation by the Pad‐decarboxylation system in Aspergillus niger, listed by increasing molecular mass, Mr. data cited are the detected GCMS peak areas of the corresponding putative product. Spores indicate conversion by whole conidia, detected from 1 mM substrate concentrations after 10 h. Cell free indicates conversion by 6-hour-induced cell free extracts obtained after 24-hour incubation. Headspace samples were adjusted to maximise sensitivity without overloading GCMS peaks, preventing quantitative comparison of conidia/cell-free extracts. This work was funded by a Defra/BBSRC Link award (FQ128, BB/G016046/1, awarded to D.B.A.) in conjunction with GlaxoSmithKline, DSM Food Specialities and Mologic Ltd. “
“Figure options Download full-size image Download as PowerPoint slide Professor Niels Skovgaard died suddenly on 16th Rolziracetam of February
2012 at the age of 87, while still actively involved in the field of Food Microbiology, and as a fully-participating member and Honorary President of the International Committee for Food Microbiology and Hygiene (ICFMH) of the IUMS. Niels was born on 29th of April 1924 in Copenhagen, the son of Kristen Skovgaard, Professor of Agricultural Economy at the Royal Veterinary and Agricultural University. He graduated as a Doctor in Veterinary Medicine (DVM) in 1951, and for the first few years of his career, worked as a veterinary practitioner, including meat inspection at a slaughterhouse. From 1955 to 1965 he was employed by the Danish government meat inspection agency with his office and laboratory facilities located at the Royal Veterinary and Agricultural University in Copenhagen. Here he became Associate Professor in 1965 and full Professor in Food Microbiology and Hygiene in 1973. He retired in 1994, at the age of 70 after holding the Chair for 21 years.