In contrast, the protein levels corresponding to NorC and the FixP and FixO components of the high affinity cbb 3 oxidase were very weak after incubation

of the cells under anoxic conditions starting at the beginning of the incubation period. The latter observations might explain the limited GM6001 datasheet nitrate-dependent growth capacity of click here E. meliloti when anoxic conditions are induced starting at the beginning of the growth period. Under these conditions, cells would be trapped, without energy, and they would be unable to produce the proteins required to cope with the oxygen-limiting conditions, most likely because of the lack of energy. Supporting this hypothesis, it was reported in Pseudomonas sp. G59 that the formation of nitrate reductase and nitrous oxide reductase did not occur under aerobic or anaerobic conditions; however, nitrate reductase

and nitrous oxide reductase were produced under microaerobic incubation [39]. The latter study suggests that dependence on microaerobiosis for the formation of these reductases was attributable to an inability to produce energy anaerobically until these anaerobic respiratory enzymes formed [39]. Recent studies have shown that the soil bacterium Agrobacterium tumefaciens is unable to maintain balanced expression of denitrification CBL0137 mw genes if oxygen depletion occurs too quickly [40, 41]. Similarly, the soil bacterium P. denitrificans appears unable to effectively switch from oxic to anoxic respiration, leaving a large fraction of the cell population in anoxia without a chance to express the denitrification proteome [41].

As suggested by Nadeem and co-workers [42], “microaerobic” Immune system denitrification is an essential trait for securing an efficient transition to anaerobic denitrification. Considering that B. japonicum, which is able to grow under anoxic nitrate-respiring conditions, is a slow-growth bacterium and E. meliloti is a fast-growth bacterium, the transition from oxic to anoxic metabolism might be different in these species. Supporting this suggestion, we observed that B. japonicum cells are able to express the FixO and FixP subunits of the cbb 3 oxidase under anoxic conditions (E. Bueno, personal communication). However, as shown in this work, E. meliloti does not express the FixO and FixP proteins under anoxic conditions. A lack of the energy necessary for protein synthesis might contribute to the inability of E. meliloti to grow via nitrate respiration when cells are initially incubated anoxically. Conclusion The potential impact of denitrification by plant endosymbiotic bacteria on the emission of the greenhouse gas N2O has been poorly investigated. The results of this work demonstrate the involvement of the napA, nirK, norC and nosZ genes in the previously reported ability of E.