In five studies based on rat models, different vectors were used to express therapeutic nucleic acids (transgenes or small interfering RNAs) Dinaciclib mw in peritoneal tissue [31, 40, 55–59]. However, no method has distinguished itself as the optimal means of preventing adhesion formation [59]. Current preventive approaches range from the use of physical barriers to the administration of pharmacological agents, recombinant proteins and antibodies, and gene therapy, yet they have all failed to consistently yield satisfactory results. Single therapeutic strategies are typically unsuccessful in preventing peritoneal adhesions due to the multi-factorial nature of adhesion pathogenesis.

Extensive literature on the subject demonstrates both the complexity of the issue and the myriad resources allocated Ilomastat to this condition, yet few interdisciplinary studies have been conducted involving experts from different fields. At this time the medical community only recognizes the “tip of the iceberg” and will continue treating the condition inadequately until it is more comprehensively explored. We are in check details agreement with Hellebrekers et al. and believe that additional prospective studies must be conducted to examine adhesion formation in relation

to factors of inflammation, coagulation, and fibrinolysis. To more effectively integrate the findings of different studies, specific attention should be paid to uniformity of measurement (what, where, and when to measure) [60]. We therefore suggest a regimented O-methylated flavonoid classification system for adhesions in an effort to standardize their definition and subsequent analysis. In this way, different surgeons in different treatment centers can more effectively evaluate patients and compare their conditions to past evaluations using a universal classification system (Figure 1). This classification is based on

the macroscopic appearance of adhesions and their extent to the different regions of the abdomen. Using specific scoring criteria, clinicians can assign a peritoneal adhesion index (PAI) ranging from 0 to 30, thereby giving a precise description of the intra-abdominal condition. Standardized classification and quantification of adhesions would enable researchers to integrate the results of different studies to more comprehensively approach the treatment and management of adhesion-related pathology. Figure 1 Peritoneal adhesion index: by ascribing to each abdomen area an adhesion related score as indicated, the sum of the scores will result in the PAI. Furthermore, as asserted by other researchers [53], we must encourage greater collaboration among basic, material, and clinical sciences. Surgery is progressively becoming more dependent on the findings of research in the basic sciences, and surgeons must contribute by practicing research routinely in a clinical setting.