In the present study we employed a well established ex vivo-in vi

In the present study we employed a well established ex vivo-in vitro model of focal laser lesions in the Prexasertib chemical structure rat visual cortex and we studied membrane and firing properties of the surviving layer

2/3 pyramidal neurons. Patch-clamp recordings, performed in the first week post-injury, revealed an increased input resistance, a depolarized spike threshold as well as alterations in the firing pattern of neurons in the cortex ipsilateral to the lesion. Notably, the reported lesion-induced alterations emerged or became more evident when an exciting perfusing solution, known as modified artificial cerebrospinal fluid, was used to increase the ongoing synaptic activity in cortical slices. Conversely, application of glutamatergic or GABA(A) receptor blockers reduced the observed alterations and GABA(B) receptor blockers abolished the differences completely. All together the present findings suggest that changes in synaptic receptors function, following focal cortical injuries, can modulate membrane and firing properties of layer 2/3 pyramidal

neurons. GW4869 This previously unknown functional interplay between synaptic and membrane properties may constitute a novel cellular mechanism to explain alterations in neuronal network function and excitability following focal cortical injuries. (c) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.”
“Background\n\nWithdrawal (detoxification) is necessary prior to drug-free treatment or as the end point of long-term substitution treatment.\n\nObjectives\n\nTo assess the effectiveness of opioid antagonists to induce opioid withdrawal with concomitant heavy sedation or anaesthesia, in

terms of withdrawal signs and symptoms, completion of treatment and adverse effects.\n\nSearch strategy\n\nWe searched the Cochrane Central Register of Controlled Trials (The Cochrane Library, Issue 3, 2009), Medline (January 1966 to 11 August 2009), Embase (January 1985 to 2009 Week 32), PsycINFO (1967 to July 2009), and reference lists of articles.\n\nSelection criteria\n\nControlled studies of antagonist-induced Selleck FK228 withdrawal under heavy sedation or anaesthesia in opioid-dependent participants compared with other approaches, or a different regime of anaesthesia-based antagonist-induced withdrawal.\n\nData collection and analysis\n\nOne reviewer assessed studies for inclusion, undertook data extraction and assessed quality. Inclusion decisions and the overall process were confirmed by consultation between all authors.\n\nMain results\n\nNine studies (eight randomised controlled trials) involving 1109 participants met the inclusion criteria for the review.

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