In vitro, LN 332 promotes the attachment, spreading, scattering and migration of non tumorigenic epithelial cells. LN 332 also stimulates human tumour cells to form lamellipodia, www.selleckchem.com/products/Imatinib-Mesylate.html leading to enhanced cell migration and invasion. Immunohisto chemical studies have shown that LN 332 is highly expressed in various types of human cancers. In particular, the laminin gamma 2 chain is expressed in tumour cells at the invasion front or in budding tumour cells in many types of human cancers such as adenocarcinoma of the colon, breast, pancreas and lung, squamous cell carcinoma and melanoma. Because endometriosis is characterised by the acquisi tion of malignant properties, such as the ability to invade surrounding tissue and disseminate to ectopic sites, Inhibitors,Modulators,Libraries the aim of the present study was to investigate the expres sion of the laminin gamma 2 chain in the tissues of women with and without endometriosis.

Methods Sample collection Endometriotic lesions were removed from Inhibitors,Modulators,Libraries women undergoing laparoscopy for pain or infertility. All the women had normal documented ovulatory cycles as well as normal endocrine parameters and did not receive hormone therapy or take oral contraception for at least 3 months before surgery. Simultaneously, eutopic endo metrium was obtained from the same women. Normal endometrial tissues were collected from healthy non menopausal women with spontaneous, regular men strual cycles who were undergoing laparo scopic surgery for benign gynaecologic indications. All endometrial biopsy samples Inhibitors,Modulators,Libraries were obtained with a Cornier Pipelle suction curette, which allows sampling of the functional layer of the endometrium.

All samples were classified according to classical histologic criteria. Patients provided informed consent, and the Institu tional Review Board of the University of Li��ge approved the collection and use of human tissue. Reverse transcription polymerase chain reaction analysis For gene expression analysis, endometrial biopsy speci mens were collected Inhibitors,Modulators,Libraries from healthy volunteers and from endometriosis patients. After surgical resection, the tissue samples were immedi ately frozen in liquid nitrogen. The frozen tissues were processed as previously described. RT PCR was performed using 10 ng aliquots of cDNA, Taq polymerase and 5 pmol of each primer. The specific PCR products were resolved on 10% polyacrylamide gels and analysed with Inhibitors,Modulators,Libraries a luminescent image analyser after GelStar staining.

The LAMC2 mRNA levels were expressed as ratios of the 28S rRNA as previously reported. Immunohistochemistry Tissue samples were fixed in 4% formalin for 4 12 hours, embedded in paraffin and cut into 4 um sections. The sections were mounted on SuperFrost Plus glass slides, dewaxed in xy lene, rehydrated and subsequently selleck chemicals llc autoclaved for 11 min at 126 C and 1. 4 bar in Target Retrieval Buffer or treated with proteinase K.