Methods: HDV-RNA, HBV, and HBsAg levels were measured every 6h du

Methods: HDV-RNA, HBV, and HBsAg levels were measured every 6h during the first day, at days 2, 3 and 7 and every 4 weeks until week 28 in 13 patients treated with pIFN-2a for up to 240 weeks. Mathematical modeling was applied to the changes

in both virus and antigen. Belnacasan ic50 Results: After initiation of therapy, a median delay of 8.5 days (interquartile range [IR]: 5.3 to 14.7 days) was observed with no significant change in HDV levels. Thereafter, HDV declined in a biphasic manner, with a rapid 1st phase lasting for 25 days (IR:23;58) followed by a slower (or plateau) 2nd phase. We previously showed a strong association between the 2nd phase in HDV and HBsAg kinetics. A mathematical model was developed that explains the biphasic HDV kinetics and assumes that the production of HDV is from HBsAg-infected cells. The model predicted that the main effect of pIFN was to block HDV production and/or release with a median effectiveness of 96% (IR:[93;99.8]). Median HDV half-life (t1/2) was estimated to be 2.9 days (IR:[1.5;5.3]) with median pretreatment production and clearance of about 1 01 0 (IR:[ 1 07-1 01 0]) virions per day. HBsAg kinetics

paralleled the 2nd phase in HDV, suggesting MK-8669 datasheet that HBsAg-productive infected cells were the source of HDV production and the median estimated loss/death rate of HDV-pro-ductive infected cells, delta=0.0051 /day (IR:[0.0015-0.035]), corresponding to a median t1/2=135 days. Three patients reached SR, defined as lack of detectable HDV RNA 6 months after completion of treatment, 2 of whom had a rapid second phase of viral decline (delta>0.04 /day), about 10 times greater than patients who did not achieve SR. Notably, no patient with a flat 2nd phase in HDV viremia (or delta~0.001 /day) reached SR. Conclusions: The new dual model of HDV and HBsAg suggests that IFN acts by blocking production/release of HDV i.e., allowing clearance of infected cells. The low estimated

the loss/death of HDV-infected cells (delta) selleck chemical explains the modest SR rate with IFN therapy. The observation that a flat 2nd phase in HDV and HBsAg kinetics was associated with non-SR provides the basis to develop early stopping rules during pIFN treatment in HDV patients. Disclosures: Jeremie Guedj – Consulting: Gilead; Grant/Research Support: Novartis Scott Cotler- Speaking and Teaching: Genentech, Vertex, Brystal Myers, Gilead Harel Dahari – Consulting: Roche TCRC, Inc The following people have nothing to disclose: Yaron Rotman, Peter Schmid, Jeff Albrecht, Vanessa Haynes-Williams, T. Jake Liang, Jay H. Hoofnagle, Theo Heller Background.Fibrosis-regression rate in treated chronic hepatitis B (CHB) patients was similar using Fibrotest (Biopredictive) or liver biopsy, while for liver stiffness measurements (LSM) by Fibroscan(Echosens) there was a possible overestimation related to necroinflammatory activity (NIA)(AVT 201 0). Aim.

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