Mixtures of all three of these strains led to cells having mixed inclusions containing the two IncA-positive strains, and a set of isolated inclusions containing the IncA-negative F(s)/70 strain (Figure 2). While progeny with each
possible combination of two antibiotic resistance markers were routinely identified in the three-way crosses, no triply resistant strain was recovered in any HKI-272 ic50 IWP-2 molecular weight experiment. Additionally, no single progeny strain had sequence at any informative position from each of the three parents in a three-way cross. Table 1 Phenotypes of parents and progeny in recombinant crosses MIC (μg/ml) Phenotype Cross Progeny Parental strains OmpA Rif Ofl Tet Plasmid Inclusion fusion Attachment 2° inclusion formation 1 RC-J/6276tet J 8 0.5 8 – + – 2 L2-434tet-13 L2 0.008 16 8 + + + 1
J/6276rif AZD6738 price J 8 0.5 0.032 – + – 1 2 RC-F(s)/342 F 32 0.5 8 – - – N/A RC-J/6276tet-rif J 8 0.5 8 – + – 1 F(s)/70rif F 32 0.5 0.032 – - – N/A 3 RC-L2(s)/46 L2 32 16 0.032 + – + N/A L2-434ofl L2 0.008 16 0.032 + + + 1 F(s)70rif F 32 0.5 0.032 – - – N/A 4 RC-F/69 F 32 4 0.032 + + + 1 L2-434ofl L2 0.008 16 0.032 + + + 1 F(s)70rif F 32 0.5 0.032 – - – N/A 5 RC-L2(s)/3 L2 32 4 0.032 – - + N/A L2-434ofl L2 0.008 16 0.032 + + + 1 F(s)70rif F 32 0.5 0.032
– - – N/A 6 RC-L2/55 L2 32 4 0.032 – + + N/A L2-434ofl L2 0.008 16 0.032 + + + 1 F(s)/70rif F 32 0.5 0.032 – - – N/A 7 RC-J/953 J 8 16 0.032 + + + 4 L2-434ofl L2 0.008 16 0.032 + + + 1 RC-F(s)/343tet-rif F 32 0.5 8 – - – N/A J/6276rifR J 8 0.5 0.032 – + – 1 8 RC-J/943 J 8 16 0.032 + + + 1 L2-434/ofl L2 0.008 16 0.032 + + + 1 RC-F(s)/343tet-rif F 32 0.5 8 – - – N/A J/6276rif J 8 0.05 0.032 – + – 1 9 RC-J/966 J 8 16 0.032 + + + 1 L2-434/ofl L2 Docetaxel in vivo 0.008 16 0.032 + + + 1 RC-F(s)/343tet-rif F 32 0.5 8 – - – N/A J/6276rif J 8 0.5 0.032 – + – 1 10 RC-L2/971 J 8 16 0.032 + + + 4 L2-434/ofl L2 0.008 16 0.032 + + + 1 RC-F(s)/343tet-rif F 32 0.5 8 – - – N/A J/6276rif J 8 0.5 0.032 – + – 1 11 RC-F(s)/852 F 32 4 8 + – - N/A RC-F/69 F 32 4 0.032 + + + 1 RC-F(s)/343tet-rif F 32 0.5 8 – - – N/A 12 RC-J(s)/122 J 32 4 8 – - + N/A RC-L2(s)/3 L2 32 4 0.032 – - + N/A RC-J/6276tet-rif J 8 0.5 8 – + – 1 Plasmid phenotype tests for presence and genotype of plasmid.