Ten customers with chronic apical periodontitis and 5 healthier controls were enrolled in this research. Based on the inflammatory cell infiltration, the specimens had been split into 2 groups serious infection group and moderate irritation group. The expression of Wnt5a was calculated by real-time PCR (RT-PCR) and immunohistochemical evaluation within the lesions of persistent apical periodontitis. The quantity of Wnt5 phrase was assayed and compared in numerous inflammation amounts. Analytical analysis ended up being carried out utilizing SPSS 13.0 software package. Wnt5a ended up being recognized in both teams. Expression of Wnt5a mRNA in patients had been substantially more than the controls (P<0.05). Based on swelling degree, the good expression rate of Wnt5a in severe swelling group was considerably more than the controls (P<0.01), and Wnt5a positive phrase in mild infection group has also been somewhat greater than the controls (P<0.05). The appearance of Wnt5a had been considerably different between extreme inflammation team and moderate irritation group (P<0.05). Thirty five OLP clients and 10 healthier volunteers were enrolled in this study sirpiglenastat in vitro . Immunohistochemical S-ABC had been utilized to detect Bcl-2 and Bax appearance in OLP team and control group. TUNEL and circulation cytometry were used to examine the apoptosis for the epithelial cells. Transmission electron microscope (TEM) was utilized to observe the modifications of ultra-microstructure of oral membrane layer of OLP. The info was reviewed with SPSS 17.0 software package. The phrase of Bcl-2 in OLP group had not been significantly not the same as control group (P>0.05), nevertheless the appearance was remarkably elevated in lymphocyte infiltration zone. Bax positive phrase rate and intensity in OLP group dramatically increased compared with the control group (P<0.01). The apoptosis index had been remarkably increased into the epithelial cells in OLP group compared to the control team (P<0.01), which was in line with the result of flow cytometry(the apoptotic percentage of this epithelial cells in OLP group was significantly more than the control group). TEM showed that the apoptotic cells of mucous membrane layer of OLP enhanced, with chromatin margination, and apoptotic human body. In 11 customers (8 with ameloblastomas, 1 with ossifying fibroma, 2 with carcinoma of the mandibular gingiva ), three-dimensional digital technology was simulated with computer software. The osteotomies were translated into quick prototyping guides. The solid model of the mandible and also the surgical guides were the same as the full dimensions and the shape, and made by using fast prototyping machine. During procedure, the bridging plate might be pre-bended in the repaired mandibular design. One group resected the diseased mandibular based on the style of the osteotomy that has been prepared before operation, one other team used auxiliary guide for precise osteotomy associated with the fibula bone with contact pedicle. The fibular portions had been reshaped and fixed with prefabricated titanium plate, and transplanted to the defect for vascular anastomosis. All of the bone flaps and osteocutaneous flaps survived. During operation, the fibula flap could be cut in proper length. Cutting, renovating and reposition of the fibula might be accelerated by surgery guides. Postoperative followup had been 1 to 24 months. Imaging evaluation showed that the shape of mandible and mandibular position were good, while the temporomandibular joint and occlusion returned to normal oral oncolytic . Twenty patients with reasonable or extreme chronic periodontitis had been selected as experimental team and 20 healthy individuals were included as control group. The patients’ baseline information, probing level (PD) and medical accessory level (CAL) had been recorded. Their particular serum and GCF were gathered then the concentration of IL-35 in GCF and serum had been recognized by ELISA. SPSS19.0 software had been employed for statistical analysis. The focus of IL-35 in GCF and serum of persistent periodontitis were significantly greater than the control team (P<0.001). There was considerable negative correlation between IL-35 and medical parameters. Sixty of maxillary anterior teeth with single-canal were collected and arbitrarily divided into 6 teams. Group a was control group, group B ended up being willing to F3 with nickel titanium ProTaper by device Protein Biochemistry , team C was ultrasonic irrigated with 3% hydrogen peroxide solution for 1 minute after planning, group D had been ultrasonic irrigated with koutai mouthwash for 1 min after planning, group E had been ultrasonic irrigated with 17% EDTA solution for 1 min after planning, team F ended up being ultrasonic irrigated with distilled water for 1 minute after preparation. The origins were then sectioned horizontally into 3 parts, split longitudinally into halves and analyzed under a micro Vickers hardness test machine. The data had been reviewed by one-way ANOVA and t test with SPSS 17.0 program. The micro-hardness of team an ended up being (52.66 ± 1.64) HV,(52.08 ± 1.53)ot canal reduce ultrasonic irrigation with. Ultrasonic irrigation with 3% hydrogen peroxide could make the micro-hardness of this apical 3rd of root channel decrease. Ultrasonic irrigation with Koutai mouthwash and distilled liquid for 1 min do not have influence on the micro-hardness of root canal.