We observed a mixed expression pattern amongst the low grade to high grade samples. Few of the high grade tumors expressed high levels of PTCH1 in the absence of GLI1 expression check FAQ and few of them showed high expression in the presence of GLI1 expression. Overall, the pattern of PTCH1 expression was low amongst all samples, despite varying levels of GLI1 expression. Cyclin D2 Silencing GLI1 in the Daoy cell line resulted in a 17% decrease in Cyclin D2 expression compared with scrambled siRNA and untransfected cell lines. We evaluated Cyclin D2 expression in 6 cell lines and 14 medulloblastoma tumor samples and observed that most of them showed high expression levels of Cyclin D2, with the exception of cell line SK PN DW and one tumor sample. We did not observe any significant correlation between GLI1 and Cyclin D2 expression.

Six astrocytoma cell lines expressed Cyclin D2 at very low levels, and two cell lines did not express Cyclin D2 compared to normal adult brain tissue. Fourteen high grade astrocytomas were assessed for Cyclin D2 and GLI1 expression only 2 glioblastomas co expressed Cyclin D2 and GLI1 at high levels. Low levels of GLI1 were found to be associated with high expression levels of Cyclin D2 in the glioblastoma samples. Plakoglobin We observed a 30% decrease in expression of Plakoglo bin in upon silencing of GLI1 in Daoy transfected cells. Most of the 6 cell lines and 14 primary tumor samples analyzed showed high expression levels of Plakoglobin compared to normal brain tissue.

Additionally, we detected an inverse correlation in levels of expression of GLI1 and Plakoglobin in primary medulloblastoma samples, with the exception of two tumors. However, this correlation was not significant. Among the 8 astrocytic cell lines, 5 showed low levels of Plako globin expression, and the remaining 3 expressed Plakoglobin at levels higher than seen in normal adult brain tissue. A majority of the astrocytic tumor samples expressed Plakoglobin at low levels com pared to normal brain tissue. There was a distinct pattern of Plakoglobin expression in astrocytic tumor samples low grade samples did not express Plakoglobin and few high grade samples highly expressed Plakoglobin in the absence of GLI1 mRNA expression. The remaining samples expressed Plakoglo bin at low levels in the presence of GLI1.

PAX6 Our results show a 35% reduction in expression of PAX6 gene upon GLI1 silencing in the Daoy cell line compared with scrambled and untransfected controls. In our study, we monitored the expression of the PAX6 gene in 6 medulloblastoma cell lines and 14 medulloblastoma primary tumor samples a majority of the cell lines showed moderate expression levels of PAX6 with the exception of the PFSK 1 and D283 cell lines. Similarly, most of the primary tumor samples showed Brefeldin_A high levels of PAX6 expression compared to GLI1 in the same samples.