Several transgenes containing GFP-tagged synaptic proteins were previously described: nuIs321 (punc-17::mCherry), nuEx379 (pacr-2::GFP), nuIs152 (punc-129::GFP::SNB-1), nuIs159 (punc-129::SYD-2::YFP), nuIs169 (punc-129::GSNL-1::YFP) ( Sieburth et al., 2005 and Sieburth check details et al., 2007), zdIs5 (pmec-4::GFP) ( Pan et al., 2008), akIs38 (UNC-29::GFP), ( Francis et al., 2005) and nuIs283 (pmyo-3::UNC-49::GFP) (J. Bai and J.M.K., unpublished
data). For real-time PCR experiments, late L4 and early adult worms were transferred to mock treatment plates or plates containing 1 mM aldicarb for 1 hr after which the RNA from these animals was harvested and quantitative PCR performed as detailed in Simon et al. (2008). This work was supported by grants from the NIH (NS32196 to J.M.K. and NS32057 to G.G.). We thank the following for strains, reagents, and advice: C. elegans Genetic Stock Center, Villu Maricq, Jihong Bai, Ed Pym, and Eyleen O’Rourke. We also thank members of the Kaplan laboratory for suggestions and comments on this manuscript. “
“Many brain functions, including memory formation and acquired neuroprotection, are controlled by transient increases in the intracellular calcium concentration induced by synaptic activity (Hardingham
and Bading, 2010 and Silva et al., 1998). Calcium can act locally near the site of entry to switch on signaling mechanisms that modulate several biochemical processes that in turn lead to changes in neuronal excitability and/or the efficacy of synaptic transmission (Bliss selleck et al.,
2007). The long-term maintenance of such activity-induced, functional adaptations requires that calcium transients invade the cell nucleus and activate or repress gene Metalloexopeptidase expression (Hardingham and Bading, 2010 and Greer and Greenberg, 2008). Nuclear calcium is one of the most potent signals in neuronal gene expression and represents a key player in the dialog between synapse and nucleus (Zhang et al., 2009). It controls cAMP response element binding (CREB)- and CREB-binding protein (CBP)-mediated transcription (Hardingham et al., 1997, Hardingham et al., 1999, Hardingham et al., 2001, Chawla et al., 1998 and Hu et al., 1999) and is critical for the acquisition of memories and the build-up of neuroprotective activity in synaptically activated neurons (Limbäck-Stokin et al., 2004, Papadia et al., 2005, Zhang et al., 2007 and Zhang et al., 2009). A picture of how genomic events induced by nuclear calcium signaling regulate persistent neuroprotection is emerging (Zhang et al., 2009, Zhang et al., 2011 and Lau and Bading, 2009). In contrast, nuclear calcium-regulated processes required for memory formation are unknown. Here we considered the possibility that nuclear calcium signaling modulates structural features of neurons, in particular the complexity of the dendritic arbor, that determine their ability to receive and process inputs (Cline and Haas, 2008).