A total of four isolates (M1, M2, M3, M4) were obtained from four out of 106 fish samples using selective agar, and identified to Mycobacterium genus using acid-fast staining and 16s rRNA gene-based genus specific polymerase chain reaction. DNA sequencing and NCBI-BLAST analysis further identified
isolate M1 as M. marinum and isolates M2, M3, M4 as M. fortuitum. Morphological, physiological and biochemical tests were carried MG-132 supplier out for phenotypic characterizations. Universal M13 and wild-type phage M13 RAPD dendogram was generated to illustrate the genetic relationship of the isolates and reference strains. (C) 2010 Elsevier Ltd. All rights reserved.”
“Severe fever with thrombocytopenia syndrome (SFTS) associated with severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging infectious disease. 12 patients with severe fever with thrombocytopenia syndrome in our study were presented mainly with fever and severe malaise. The clinical manifestations typically became worse on the 6th or 7th day. The average fever time is 9.11 +/- 1.54 days. Most of them had multiorgan dysfunction, and part of them had hemophagocytic lymphohistiocytosis histiocytosis (HLH). The characteristic laboratory findings in the early stage were the drop of white blood cells (WBC), platelets (PLT) and serum Ca++, while increase of aspartate amino transferase
(AST), creatine kinase (CK), and lactate dehydrogenase (LDH). CD3+CD4+ were significantly decreased, while CD3-CD56+ were significantly Angiogenesis inhibitor increased, whereas CD3+CD8+ were constantly elevated throughout the disease course. Ten to 14 days after illness onset, symptoms were improved, accompanied by resolution of laboratory abnormalities. These results indicate that severe fever with thrombocytopenia syndrome has an acute onset and self-limited course. It is a systemic infection. The host immune response caused tissues and
organs injury. The improvement of symptoms and laboratory tests is consistent with the elimination of the virus and recover of immune response. Further investigation should be done in order to better understand this disease and guide the clinical treatment. (C) 2013 GSK2399872A research buy Elsevier Editora Ltda. All rights reserved.”
“We report a theoretical study of the equilibrium spin current (ESC) flowing in a ferromagnet/ferromagnet (FM/FM) graphene junction with noncollinear magnetizations, in which the FM correlation in graphene is induced by depositing an FM insulator on it. Using the scattering matrix method, we derived an analytical expression of the ESC which is formally the same as that of a normal FM/FM junction. The ESC comes from the exchange coupling between the two FM magnetizations. Owing to the linear dispersion of graphene, the ESC disappears in the one-dimensional case, while it exists in the two-dimensional case. It exhibits damped oscillations with change in the Fermi energy, the layer length between the two FM regions, and the FM exchange splitting.